Intermediates for preparing semi-synthetic penicillins and processes relating thereto

ABSTRACT

Phosphorylated penicillins and cephalsporins are described which are obtained by reacting a penicillin or cephalo porin with a trivalent or pentavalent phosphorus halide and treating the thus formed product sequently with an acid chloride, an alcohol and water to form a phosphorylated anhydride of a 6-aminopenicillanic acid or 7-aminocephalosporanic acid salt, respectively, and thereafter acylating the thus formed product and splitting off the carboxyl protective group to obtain the desired penicillin or cephalosporin compound.

This application is a continuation-in-part of copending application Ser. No. 186,397 filed Oct. 4, 1971 now U.S. Pat. No. 3,896,110.

This invention relates to novel phosphorylated penicillin and cephalosporin derivatives and processes relating to their preparation and use in manufacturing semi-synthetic penicillins and cephalosporins.

In its broadest aspects the present invention covers compounds containing the following chemical structure: ##SPC1## ##SPC2##

Z and Z' are each an organic radical and may be joined together to form a heterocyclic ring with the phosphorus atom; Y is hydrogen or an acyl radical. These compounds are useful as intermediates to produce non-toxic cephalosporins and penicillins exhibiting gram positive and/or gram negative activity against microorganisms. In its more narrow aspects the invention provides compounds of the formulae ##SPC3##

Wherein:

R is selected from the class consisting of hydrogen, (lower)alkanoyloxy containing 2 to 8 carbon atoms (e.g. acetoxy), propionoyloxy, butanoyloxy, etc.), aryloxy, a quaternary ammonium radical (e.g. pyridinium, quinolinium, picolinium, etc.); R³ is oxygen (=O) when the phosphorous atom is pentavalent; R⁵ is selected from the class consisting of hydrogen and an organic acyl group; R¹ and R² are selected from the class consisting of (lower)alkoxy, (lower)alkylthio, aryloxy, arylthio, aryl(lower)alkyloxy, aryl(lower)alkylthio, halogen, (lower)alkyl, aryl, aryl(lower)alkyl, halo(lower)alkyloxy, halo(lower)alkyl and a radical of the following structure: ##SPC4##

Preferably, R¹ and R² are joined together to form with the phosphorus atom the ring: ##SPC5##

n is an interger from 0 to 1; m is an interger from 1 to 6; X is selected from the class consisting of sulfur; oxygen and methylene; R⁴ is selected from the class consisting of hydrogen and from one to three (lower)alkyl radicals; X is preferably oxygen.

The term "(lower)alkyl" is employed herein alone or in conjunction with other designated groups is intended to encompass straight chain or branch chain alkyl groups consisting of from one through six carbon atoms (e.g. methyl, ethyl, propyl, butyl, isobutyl, hexyl, 2-ethylpropyl, etc.).

The term "(lower)alkoxy" is employed herein alone or in conjunction with other designated groups is intended to encompass straight chain or branched chain alkoxy groups having one through six carbon atoms (e.g. methoxy, propoxy, butoxy, isobutoxy, pentoxy, 1,1-dimethylbutoxy). The term "halogen" as used herein is intended to encompass chlorine, bromine, iodine and fluorine. The term "aryloxy" encompasses phenoxy, naphthoxy, as well as unsaturated heterocyclic rings containing 5 to 7 ring members and from 1 to 3 hetero atoms selected from the class consisting of nitrogen, oxygen and sulfur. The term "aryl(lower)alkyloxy" encompasses an aryl nucleus linked through a ring carbon atom to a (lower)alkyloxy group. Illustrative of aryl(lower)alkyloxy are benzyloxy, phenethyloxy, etc. The term "aryl" is illustrated by phenyl and naphthyl and the term "aryl(lower)alkyl" is illustrated by benzyl, phenethyl, etc. Illustrative of (lower)alkylthio are thiomethyl, thiobutyl, thioisopropyl, etc. Illustrative of "arylthio" are phenylthio, chlorophenylthio, methylphenylthio, methoxyphenylthio, etc. Illustrative of "aryl(lower)alkylthio" are benzylthio, phenethylthio, chlorophenethylthio, etc.

The acyl group defined by R⁵ is derived from an organic carboxylic acid or organic sulfonic acid or a suitable reactive functional derivative thereof.

The preferred acyl groups defined by R⁵ are selected from groups having the following formulae wherein those having a free amino group are in the form of an acid addition salt which may subsequently be converted to the free base. ##SPC6##

wherein:

R⁶ and R⁷ are selected from the group consisting of hydrogen and (lower)alkoxy;

R⁸ is selected from the group consisting of hydrogen, (lower)alkyl and phenyl;

R⁹ and R¹⁰ are selected from the group consisting of hydrogen and halogen;

R¹¹ and R¹² are selected from the group consisting of hydrogen, halogen, (lower)alkyl, (lower)alkoxy, phenyl and phenoxy;

R¹³ is selected from the class consisting of hydrogen, (lower)alkyl and aryl;

a is an interger from 0 to 1; b is an integer from 0 through 5;

c is an interger from 0 to 2; d is an interger from 1 through 3, with the proviso that when a is 0, d is greater than 1, and when a is 1, d is less than 3.

The process of the present invention is illustrated by the following flow diagram: ##SPC7## ##SPC8##

In the foregoing reaction sequence M is selected from the class consisting of hydrogen, alkali metal (e.g. Na, K. etc.) and a tertiary amine (e.g. triethylamine, dimethylaniline) and R¹⁵ is a substituent selected from those contained in natural and synthetic penicillins. As used herein the term "natural penicillins" include those which are produced by fermentation as well as those that are biosynthetically prepared by the addition of certain precursors to the fermentation broth. Examples of these penicillins are: Penicillin S (R¹⁵ is -chlorocrotylmercaptomethyl); Penicillin V (R¹⁵ is phenoxymethyl); Penicillin G (R¹⁵ is benzyl); Penicillin F (R¹⁵ is 2-pentyl); Penicillin K (R¹⁵ is 2-heptyl); Penicillin X (R¹⁵ is P-hydroxybenzyl); Dihydro Penicillin F (R¹⁵ is amyl); Penicillin T (R¹⁵ is p-aminobenzyl); Penicillin O (R¹⁵ is allylmercaptomethyl); Penicillin N (R¹⁵ is 4-amino-4-carboxybutyl). Although it is preferred to use a natural penicillin as the starting material of formula I for reaction with a phosphorus halide of formula II, the process is not limited to operability to these starting materials and will operate utilizing a synthetic penicillin as a reactant within the scope of formula I.

Illustrative of specific phosphorus halides falling within the scope of formula II are phosphorus oxychloride, phosphorus trichloride, diphenyl phosphorochloridate, phosphorochloridate, phenyl phosphorodichloridate, ethyl phosphorodichloridate, cyclic ethylene phosphorochloridate, diethyl phosphorochloridate, diethyl phosphorochloridite, ethyl phosphorodichloridite, cyclic ethylene phosphorochloridite (2-chloro-1,3,2-dioxaphospholane), cyclic propylene phosphorochloridite (2-chloro-1,3,2-dioxaphosphorinane), etc.

The reaction of a compound of formula I with a compound of formula II is preferably carried out in the presence of an anhydrous inert aprotic solvent and acid binding agent. This reaction is carried out at a temperature in the range of about -40° to about +10°C., preferably -10°C. to about +10°C. The molar ratio of a compound of formula I to a compound of formula II is about 0.5:1 to 3:1, preferably about 1:1. Where the molar ratio is greater than 1:1 and at least one of R¹ or R² is halogen, dimers or trimers can be obtained as illustrated by the following formulae: ##SPC9##

Suitable acid binding agents are tertiary amines such as triethyl amine, dimethylaniline, quinoline, pyridine, lutidine, alkali metal carbonates; alkaline earth carbonates or other acid binding agents known in the art. The preferred acid binding agent is a week tertiary amine. As used herein "strong amines" are those characterized by having dissociation constants in the range of from 10⁻ ³ to 10⁻ ⁶ or having comparable basicity, as distinguished from "weak amines" which are characterized by having dissociation constants in the range of from 10⁻ ⁸ to 10⁻ ¹¹.

A wide range of anhydrous non-hydroxylic organic solvents are useful in the reaction of a compound of formula I with a phosphorylating agent including hydrocarbons such as toluene; chlorinated solvents such as methylene chloride, chloroform and chlorobenzene; ethers such as diethyl ether, tetrahydrofuran; and other conventional solvents such as methylisobutylketone, dimethylformamide, ethyl acetate, acetonitrile, etc., the preferred solvents being the chlorinated solvents.

The carboxy protected penicillin represented by formula III is converted to the corresponding imino halide represented by formula IV by reaction with an acid chloride in the presence of an acid acceptor. Examples of suitable acid halides includes phosphorus pentachloride, phosgene, phosphorus oxychloride, oxalyl chloride, p-toluene sulfonic acid chloride, phosphorus pentabromide, etc. The acid acceptor is an acid binding agent which may be any one of those previously described in connection with the reaction of a compound of formula I with a compound of formula II. The formation of the amino halide compound is carried out under anhydrous conditions at temperatures below 0°C., preferably between -10°C. and -65° C. An inert organic solvent such as methylene chloride, dichloroethane, chloroform, diethyl ether, or any other solvent previously described herein is present during the reaction. Since it is not necessary to isolate the intermediate of formula III (although this may be done), the solvent used in reacting a compound of formula I with a compound of formula II is present when a compound of formula III is reacted with an acid halide. A slight excess of acid halide, not more than about 10%, is used in the formation of a compound of formula IV.

The imino halide compound of formula IV is converted to the imino ether hydrohalide of formula V by reacting the imino halide under anhydrous conditions with a primary or secondary alcohol (R^(a) OH) at temperatures below about -10°C., preferably between about -30°C. and -60°C. At these temperatures, the carboxyl protecting group is resistant to splitting off. Illustrative of R^(a) is lower alkyl, hydroxy(lower)alkyl, phenyl(lower)alkyl, cycloalkyl of 4 to 8 carbon atoms, (lower)alkoxy(lower)alkyl, aryloxy(lower)alkyl, etc.

Examples of suitable primary and secondary R^(a) OH compounds are methanol, ethanol, n-butanol, isopropanol, amyl alcohol, benzyl alcohol, 2-phenylethanol-1, cyclohexyl alcohol, 1,6 hexanediol, 2-methoxyethanol, 2-isopropoxyethanol, 2-butoxyethanol, 2-p-chlorophenoxyethanol, 2-(p-methoxybenzyloxy)-ethanol, diglycol, etc.

The imino ether bond is split without removal of the carboxyl protecting group. This is accomplished by hydrolysis with water at a temperature below -20°C., preferably the temperature is between -30°C. and -60°C.

After formation of a compound of formula VI, acylation is carried out at a temperature between -60°C. and room temperature, preferably below 0°C. in the presence of an acid binding agent which may be one of materials previously described, to produce a compound of formula VII. The acid binding agent is preferably a weak amine. Sufficient acid binding agent must be employed to remove the hydrogen halide from a compound of formula VI, and permit immediate acylation of the 6-amino group. In carrying out this reaction an excess of acylating agent to acid acceptor is employed.

Suitable acylating agents include carboxylic acid halides, carboxylic acid anhydrides, mixed anhydrides with other carboxylic or inorganic acids; esters such as thiol esters and phenol esters; lactones; and carboxylic acids with carbodiimides or N,N¹ -carboxyldiimidazoles.

Illustrative of some specific preferred acylating agents are phenoxyacetyl chloride, 2,6-dimethoxybenzoyl chloride, benzene sulfonyl chloride, 2-phenoxypropionyl chloride, 2-phenoxybutryl chloride, D(-)phenylglycyl chloride HCl, 1-aminocyclopentanecarboxylic acid chloride HCl, 1-aminocyclohexanecarboxylic acid chloride HCl, 2-amino-2-carboxyindane acid chloride HCl, 2-ethoxy naphthoyl bromide and 3-(2,6-dichlorophenyl)-5-methyl-isoxazole-4-carbonyl chloride, etc.

The acylated penicillin of formula VII is obtained as the hydrohalide salt if the acyl radical contains an α or β-amino group. This intermediate of formula VII is converted to a semi-synthetic penicillin compound of formula VIII by hydrolysis to remove the carboxyl protecting group. If this product is in the form of a hydrohalide salt, it is readily converted to its corresponding basic form by neutralization processes which are well known to those skilled in the art of chemistry. For example, the hydrohalide salt is treated for a short time with water containing a base, such as triethylamine, sodium bicarbonate or the like, preferably in the presence of a water-miscible solvent.

In the event the ultimate penicillin to be obtained is α-aminobenzyl penicillin (ampicillin), it has been found advantageous to change the halide to an aryl sulfonic acid salt of the aminopenicillin either by adding an appropriate sulfonic acid to the reaction mixture comprising the selected organic solvent and water, or to the aqueous extracts separated as described immediately above. In this connection, a 25% excess of the sulfonic acid has been used to advantage in preparing the corresponding salt of ampicillin.

The aryl sulfonic salt of the α-aminobenzyl penicillin may then be converted to the penicillin per se by reaction with a base such as triethylamine or diethylamine in approximately 85% isopropanol. In the case of ampicillin specifically, the sulfonic acid salt, wet with water and ethyl acetate, may be added to isopropanol containing a molar equivalent of triethylamine at 75°-80°C., whereby the anhydrous form of ampicillin described and claimed in U.S. Pat. No. 3,144,445 is formed and collected by filtration from the hot mixture.

Alternatively, the corresponding penicillin may be obtained, but in hydrated form, by raising the pH of the aqueous reaction mixture containing the hydrochloride salt of said penicillin to the iso-electric point.

In place of a penicillin of formula I, a cephalosporin of the following formula can be used to produce semi-synthetic cephalosporins ##SPC10##

wherein R and M have the same meaning as previously defined and R¹⁶ is a substituent selected from those contained in natural and synthetic cephalosporins as exemplified by the numerous examples in the prior art. Illustrative of substituents from natural cephalosporins are 2-thienylmethyl, (Cephalothin); 4-amino-4-carboxy-n-butyl, (Cephalothin C); other cephalosporin compounds are illustrated by those disclosed in U.S. Pat. Nos. 3,499,909; 3,093,638; 3,207,755.

The natural or synthetic penicillins and cephalosporins employed as starting materials are commercially available or are described in the literature. Similarly, the phosphorus halides of formula II are commercially available or in those instances where they are not available, they may be synthesized readily by standard organic procedures described in the chemical literature and known to those skilled in the art.

The following examples are given by way of illustrating some embodiments of this invention.

EXAMPLE 1 D(-)α-aminobenzylpenicillin

Potassium penicillin G (75 g., 0.2 mole) and N,N-dimethylaniline (DMA) (53 ml., 0.418 mole) are stirred in 300 ml. of dichloromethane at 10°C. while 2-chloro-1,3,2-dioxaphospholane (27 ml., 0.3 mole) is added over 20 min. The resulting product is 6-phenylacetamido penicillanic acid, 1,3,2-dioxaphospholan-2-yl ester. After stirring an additional 1/2 hr. at 10°C. the temperature is lowered to -55°C. and powdered PCl₅ (45 g., 0.216 mole) is added all at once. With continuous cooling in an acetone-dry ice bath, the temperature rises to about -45°C., and the mixture is stirred for 2 hr. at -40°C. The product obtained from this reaction is 6-[(α-chlorophenethylidene)amino]penicillanic acid, 1,3,2-dioxaphospholan-2-yl ester. The temperature is again lowered to -60°C., and 67 ml. of absolute alcohol is added at the fastest rate allowable so that the temperature may be kept below -40°C. to produce 6-[(α-ethoxyphenethylidene)amino]penicillanic acid, 1,3,2-dioxaphospholan-2-yl ester. After stirring 21/2 hr. at -40°C. the temperature is lowered to -50°C. and 52.5 ml. of water is dripped in without allowing the temperature to go above -40°C. to produce the hydrochloride salt of 6-aminopenicillanic acid, 1,3,2-dioxaphospholan- 2-yl ester. After stirring overnight (about 18 hr.) at -40°C., the flask is equipped with a dropping funnel containing 112 ml. of dimethylaniline and a 250 ml. Erlenmeyer flask containing 52.5 g. of D-(-)-phenylglycylchloride hydrochloride connected with a Gooch tube.

About 1/6 of the acid chloride is added and dimethylaniline is slowly dropped in. At this point the temperature is allowed to rise to -25°C. The two are concurrently added so that all the acid chloride is added over 30 min. and the dimethylaniline added over 40 min., with the object having to always have more acid chloride present in the mixture than dimethylaniline. After another 40 min. the reaction mixture is almost clear, the hydrochloride salt of D(-)-α-aminophenylacetamido penicillanic acid, 1,3,2-dioxaphospholan-2-yl ester is poured into 700 ml. water, and the flask rinsed with 100 ml. water to produce the hydrochloride salt of D(-)-α-aminobenzylpenicillin. The aqueous mixture is rapidly stirred in an ice bath for 15 min. Super-Cell is added, the mixture is filtered, and the cake washed with 200 ml. water. After separation of the layers, the aqueous phase is placed in a 2 liter, 4 neck flask and 150 ml. ethylacetate added. The mixture is cooled to 0°-10°C., the pH adjusted to 1.7 with 5N sodium hydroxide, and 105 ml. of 37% naphthalene sulfonic acid is added over about 10 min. at 0°-10°C. while keeping the pH at 1.5-1.7 with 5N sodium hydroxide. During the addition of the nephthalene sulfonic acid, some seeds of the naphthalene sulfonic acid salt of D(-)-α-aminobenzylpenicillin are added and the flask is scratched to hasten crystallization. Stirring is continued for 6 hr. at 0°-10°C. and the naphthalene sulfonic acid salt of D(-)-α-aminobenzylpenicillin is filtered off. It is stirred in 250 ml. of cold, pH 1.5 to 1.7 water for 5 min. and filtered. The cake is pressed and sucked fairly dry, and then stirred for 5 min. in 250 ml. of ethylacetate. The slurry is filtered and the cake washed twice with ethylacetate giving 109 g. of naphthalene sulfonic acid salt of D(-)-α-aminobenzylpenicillin with a NVM of 54.5%, and a corresponding 53% yield of naphthalene sulfonic acid salt of D(-)-α-aminobenzylpenicillin. This salt is converted to anhydrous ampicillin by the standard isopropanol procedure described in U.S. Pat. No. 3,487,073.

EXAMPLE 2 α-aminobenzylpenicillin

Following the procedure of Example 1, but using 31 ml. (0.3 moles) of 2-chloro-1,3,2-dioxaphosphorinane and 45 ml. of water the naphthalene sulfonic acid salt of 6-α-aminobenzylpenicillin is isolated in 56% yield.

EXAMPLE 3

Potassium penicillin G (75 g., 0.2 moles) and N,N-dimethylaniline (DMA) (53 ml., 0.418 moles) are stirred in 300 ml. of dichloromethane at 0°C. while phosphorus trichloride (8.8 ml., 0.1 mole) is added over 10 minutes. After stirring an additional 1/2 hour at 0°C., the temperature is lowered to -55°C. and powdered PCl₅ (45 g., 0.216 mole) is added all at once. With continuous cooling in the acetone-dry ice bath, the temperature rises to about -45°C., and the mixture is stirred for 2 hours at -40°C. The temperature is again lowered to -60°C. and 72.8 ml. of absolute alcohol containing 12.7 ml. (0.1 mole) of DMA is added at the fastest rate allowable so that the temperature could be kept below -40°C. After stirring 21/2 hours at 31 40°C. the temperature is lowered to -50° C. and 45 ml. of water is dripped in without allowing the temperature to go above -40°C. The reaction is then reacted and worked up in a manner similar to Example 1 giving the naphthalene sulfonic acid salt of 6-α-aminobenzyl penicillin in 57% yield.

The following Examples 4 through 8 are illustrative of specific carboxyl protected penicillins and cephalosporins that may be prepared in accordance with the process of the present invention.

EXAMPLE 4 ##SPC11##

wherein R¹, R², R³, R¹ +R² and n are defined as follows:

    R.sup.1    R.sup.2     R.sup.3  R.sup.1 +R.sup.2                                                                       n                                      ______________________________________                                         Cl         Cl          =0               1                                      Cl         OCH.sub.3                    0                                      OCH.sub.3  OCH.sub.3   =0               1                                      0--φ   0--φ                     0                                      0--CH.sub.2 --φ                                                                       Cl          =0               1                                      OC.sub.2 H.sub.5                                                                          OC.sub.2 H.sub.5             0                                                                      O                                              --         --                   ;∠                                                                               0                                                                      O                                                                              O                                              --         --                   ;∠                                                                               0                                                                      O                                              0--φ   0--CH.sub.2 --φ          0                                      ______________________________________                                    

EXAMPLE 5 ##SPC12##

wherein R¹, R², R₃, R¹ +R² and n are defined as follows:

    R.sup.1    R.sup.2     R.sup.3  R.sup.1 +R.sup.2                                                                       n                                      ______________________________________                                         Br         Br          =0               1                                      Cl         OC.sub.2 H.sub.5             0                                      0--φ   Cl          =0               1                                      Cl         OC.sub.2 H.sub.5                                                                           =0               1                                      OC.sub.2 H.sub.5                                                                          OC.sub.2 H.sub.5             0                                      0--CH.sub.2 --φ                                                                       0--CH.sub.2 --φ          0                                                                      O                                              --         --                   ;∠                                                                               0                                                                      O                                              ______________________________________                                    

EXAMPLE 6 ##SPC13##

wherein R¹, R², R³, R¹ +R² and n are defined as follows:

    R.sup.1    R.sup.2   R.sup.3  R.sup.1 +R.sup.2                                                                         n                                      ______________________________________                                         Cl         Cl        =0                 1                                      OC.sub.2 H.sub.5                                                                          OC.sub.2 H.sub.5                                                                         =0                 1                                      Cl         0--φ  =0                 1                                      Cl         OC.sub.2 H.sub.5             0                                      0--CH.sub.2 --φ                                                                       Cl        =0                 1                                      0--φ   0--φ  =0                 1                                                                    OCH.sub.3                                        --         --                 ;∠CH.sub.3                                                                         0                                                                    O                                                ______________________________________                                    

EXAMPLE 7 ##SPC14##

wherein R¹, R², R³, R^(1+R) ² and n are defined as follows:

    R.sup.1  R.sup.2    R.sup.3                                                                               R.sup.1 +R.sup.2                                                                             n                                     ______________________________________                                         Cl       Cl         =0                 1                                       OCH.sub.3                                                                               OCH.sub.3  =0                 1                                       0--φ OCH.sub.3                     0                                       Cl       OC.sub.2 H.sub.5                                                                          =0                 1                                       0--CH.sub.2 --φ                                                                     0--CH.sub.2 --φ           0                                       0--φ Cl         =0                 1                                       --       --                            0                                       ______________________________________                                    

EXAMPLE 8 ##SPC15##

wherein R¹, R², R³, R¹ +R² and n are defined as follows:

    R.sup.1    R.sup.2     R.sup.3  R.sup.1 +R.sup.2                                                                       n                                      ______________________________________                                         Cl         Cl          =0             1                                        OC.sub.2 H.sub.5                                                                          OC.sub.2 H.sub.5                                                                           =0             1                                        Cl         Cl                         0                                        0--φ   0--φ    =0             1                                        0--φ   0--φ                   0                                        Br         Br                         0                                        0--CH.sub.2 --φ                                                                       0--CH.sub.2 --φ                                                                        =0             0                                        ______________________________________                                    

The following tables are illustrative of other representative compounds that may be obtained in accordance with the processes described herein:

                                      Table 1                                      __________________________________________________________________________                                                        Hydrochloride Salt          Starting                                                                               Phosphorylating                                                                           Imino Halide Derivative                                                                        Imino Ether Derivative                                                                         of 6-APA Derivative         Material                                                                               Compound   of Formula IV   of Formula V    of Formula                  __________________________________________________________________________                                                        VI                          2-pentenyl                                                                             2-chloro-1,3,2-                                                                           6-[(1-bromo-3-hexenyli-                                                                        6-[(1-phenoxy-3-hexenyli-                                                                      6-aminopenicillanic         penicillin                                                                             dioxaphospholane                                                                          dene)amino]penicillanic                                                                        dene)amino]penicillanic                                                                        acid-1,3,2-dioxa-                              acid-1,3,2-dioxaphospho-                                                                       acid-1,3,2-dioxaphospho-                                                                       phospholan-2-yl-                               lan-2-yl-ester  lan-2-yl-ester  ester                       n-heptyl                                                                               diethyl phosphoro-                                                                        6-[(1-chlorooctylidene)                                                                        6-[(1-ethoxyoctylidene)                                                                        6-aminopenicillanic         penicillin                                                                             chloridate amino]penicillanic acid                                                                        amino]penicillanic acid                                                                        acid anhydride with                            monoanhydride with phos-                                                                       anhydride with phosphoric                                                                      phosphoric acid di-                            phoric acid diethyl ester                                                                      acid diethyl ester                                                                             ethyl ester                 3,5-diphenyl-                                                                          methyl phosphoro                                                                          6-[chloro(3,5-diphenyl-4-                                                                      6-[(α-ethoxy)-3,5-diphenyl-                                                              6-aminopenicillanic         4-isoxazolyl                                                                           dichloridite                                                                              isoxazolylmethylene)amino]                                                                     4-isoxazolylmethylene)                                                                         acid monoanhydride          penicillin         penicillanic acid monoan-                                                                      amino]penicillanic acid                                                                        with phosphorochlori-                          hydride with phosphoro-                                                                        monoanhydride with phos-                                                                       dous acid methyl es-                           chloridous acid methyl ester                                                                   phorochloridous acid                                                                           terhyl                                                         ester                                       benzyl peni-                                                                           diethyl phosphoro-                                                                        6-[(α-bromophenethylidene)                                                               6[(α-benzyloxyphenethyl-                                                                 6-aminopenicillanic         cillin  chloridite amino]penicillanic acid                                                                        idene)amino]penicillanic                                                                       acid monoanhydride                             monoanhydride with phos-                                                                       acid monoanhydride with                                                                        with phosphorus acid                           phorus acid diethyl ester                                                                      phosphorus acid diethyl                                                                        diethyl ester                                                  ester                                       α-phenoxyethyl                                                                   phenyl phosphoro-                                                                         6-[(1-chloro-2-phenoxy pro-                                                                    6-[(1-methoxy-2-phenoxy-                                                                       6-aminopenicillanic         penicillin                                                                             dichloridate                                                                              pylidene)amino]penicillanic                                                                    propylidene)amino]penicil-                                                                     acid monoanhydride                             acid monoanhydride with                                                                        lanic acid monoanhydride                                                                       with phosphorochlori-                          phosphorochloridic acid phe-                                                                   with phosphorochloridic                                                                        dic acid phenyl es-                            nyl ester       acid phenyl ester                                                                              ter                                                                            Hydrochloride Salt          Starting                                                                               Phosphorylating                                                                           Imino Halide Derivative                                                                        Imino Ether Derivative                                                                         of 6-APA or 7-ACA           Material                                                                               Compound   of Formula IV   of Formula V    Derivative                  __________________________________________________________________________     2-ethoxy                                                                               2-chloro-1,3,2-                                                                           6[[chloro(2-ethoxynapthyl)                                                                     6-[(ethoxy(2-ethoxy                                                                            6-aminopenicillanic         naphthyl                                                                               dioxaphosphorinane                                                                        methylene]amino]penicillanic                                                                   napthyl)methylene)amino]                                                                       acid-1,3,2-dioxa-           penicillin         acid-1,3,2-dioxaphosphorinan-                                                                  penicillanic acid-1,3,2-                                                                       phosphorinan-2-yl                              2-yl ester      dioxaphosphorinan-2-yl                                                                         ester                                                          ester                                       cephalothin                                                                            dibenzyl phosphoro-                                                                       7-[[1-chloro-2-(2-thienyl)                                                                     7-[(1-ethoxy-2-(2-thienyl)                                                                     7-aminocephalo-                     chloridate ethylidene]amino]cephalo-                                                                      ethylidene)amino]cephalo-                                                                      sporanic acid mono-                            sporanic acid monoanhydride                                                                    sporanic acid monoanhydride                                                                    anhydride with phos-                           with phosphoric acid dibenzyl                                                                  with phosphoric acid                                                                           phoric acid diben-                             ester           zyl ester       zyl ester                   __________________________________________________________________________

                                      Table 2                                      __________________________________________________________________________     Hydrochloride Salt of       Acylated Penicillin                                                                           Final Penicillin                    6-APA or 7-ACA Derivative                                                                     Acylating Agent                                                                             or Cephalosporin                                                                              or Cephalosporin                    __________________________________________________________________________     6-aminopenicillanic                                                                           benzene sulfonyl                                                                            6-(benzenesulfonamido)                                                                        6-(benzenesulfona-                  acid, 1,3,2-dioxa-                                                                            chloride     penicillanic acid, 1,3,                                                                       mido)penicillanic                   phospholan-2-yl ester       2-dioxaphospholan-2-yl                                                                        acid                                                            ester                                              6-aminopenicillanic                                                                           N-carboxyanhydride                                                                          6-(2-amino-2-phenoxy-2-                                                                       6-(2-amino-3-phen-                  acid monoanhydride with                                                                       of 2-amino-2-phenoxy-                                                                       indane carboxamido)peni-                                                                      oxy-2-indanecar-                    phosphoric acid diethyl                                                                       2-indane carboxylic                                                                         cillanic acid monoanhy-                                                                       boxamido)penicil-                   ester          acid         dride with phosphoric                                                                         lanic acid                                                      diethyl ester                                      6-aminopenicillanic                                                                           furane-2-carboxylic                                                                         6-(2-furanecarboxamido)                                                                       6-(2-furanecar-                     acid monoanhydride with                                                                       acid chloride                                                                               penicillanic acid mono-                                                                       boxamido)penicil-                   phosphorochloridous acid    anhydride with phosphoro-                                                                     lanic acid                          methyl ester                chloridous acid methyl                                                         ester                                              6-aminopenicillanic                                                                           N-carboxyanhydride                                                                          6-(2-amino-3-phenoxy-2-                                                                       6-(2-amino-3-phen-                  acid monoanhydride with                                                                       of 2-amino-2-phenoxy-                                                                       indane carboxamido)peni-                                                                      oxy-2-indane car-                   phosphorus acid diethyl                                                                       2-indane carboxylic                                                                         cillanic acid monoanhydride                                                                   boxamido)penicil-                   ester          acid         with phosphorus acid diethyl                                                                  lanic acid                                                      ester                                              6-aminopenicillanic                                                                           N-carboxyanhydride                                                                          6-(1-aminocyclopentanecar-                                                                    6-(1-aminocyclo-                    acid monoanhydride with                                                                       of 1-aminocyclopen-                                                                         boxamido)penicillanic acid                                                                    pentanecarboxamido)                 phosphorochloridic acid                                                                       tane carboxylic acid                                                                        monoanhydride with phos-                                                                      penicillanic acid                   phenyl ester                phorochloridic acid phenyl                                                     ester                                              6-aminopenicillanic acid-                                                                     D(-)phenyl glycyl                                                                           6-(α-aminophenyl-                                                                       6-(α-aminophenyl-             1,3,2-dioxaphospholan-2-                                                                      chloride hydro-                                                                             acetamido)penicil-                                                                            acetamido)penicil-                  yl ester       chloride     lanic acid-1,3,2-dioxa-                                                                       lanic acid                                                      phospholan-2-yl ester                              7-aminocephalosporanic                                                                        N-carboxyanhydride                                                                          7-(1-aminocyclohexane-                                                                        7-(1-aminocyclo-                    acid monoanhydride with                                                                       of 1-aminocyclohexane                                                                       carboxamido)cephalo-                                                                          hexaneacetamido)                    phosphoric acid dibenzyl                                                                      carboxylic acid                                                                             sporanic cid monoanhy-                                                                        cephalosporanic                     ester                       dride with phosphoric                                                                         acid                                                            acid dibenzyl ester                                __________________________________________________________________________ 

What is claimed is:
 1. A process for preparing a semi-synthetic penicillin which comprises:a. reacting a penicillin compound of the formula ##SPC16## with a phosphorylating agent of the formula ##SPC17##to produce a compound of the formula ##SPC18## said reaction being carried out under anhydrous conditions in the presence of an inert non-hydroxylic organic solvent and a tertiary amine at a temperature in the range of about -40°C to about +10°C, the molar ratio of said penicillin compound to said phosphorylating agent being about 0.5:1 to about 3:1; b. reacting said compound obtained in step (a) with an acid chloride in the presence of a tertiary amine at temperature between about -10°C and -65°C to produce an imino chloride compound of the formula ##SPC19## c. reacting said imino chloride with an alcohol at a temperature below about -10°C to produce an imino ether of the formula ##SPC20## d. reacting said imino ether with water at a temperature below about -20°C and treating the resulting compound with a reactive derivative of an organic carboxylic acid in the presence of a tertiary amine at a temperature between about 0°C and -60°C to produce a compound of the formula ##SPC21##e. and hydrolyzing the resulting acylated product to remove the carboxyl protective group and obtain a semi-synthetic penicillin; wherein: M is selected from the class consisting of hydrogen, an alkali metal and a tertiary amine; R¹⁵ is selected from the class consisting of phenoxymethyl and benzyl; R⁴ is selected from the class consisting of hydrogen and lower alkyl; R^(a) is selected from the class consisting of lower alkyl, phenyl(lower)alkyl, cycloalkyl of four through eight carbon atoms, phenoxy(lower)alkyl, (lower)alkoxy(lower)alkyl; and m is an integer from 1 to
 6. 2. A process according to claim 1 wherein said phosphorylating agent is selected from the class consisting of 2-chloro-1,3,2-dioxaphospholane and 2-chloro-1,3,2-dioxaphosphorinane.
 3. A process according to claim 2 wherein said acylating agent is D(-)phenylglycyl chloride hydrochloride.
 4. A process for preparing a semi-synthetic penicillin which comprises:a. reacting a compound of the formula: ##SPC22##with a phosphorylating agent of the formula ##EQU1## in the presence of an acid binding agent and an inert, non-hydroxylic organic solvent at a temperature between about -40°C and about +10°C to obtain a compound of the formula: ##SPC23## b. reacting an acid halide with a compound obtained in step (a) in the presence of an acid binding agent at a temperature below 0°C to produce the corresponding imino halide; c. reacting said imino halide compound with a alcohol at a temperature below about -10°C to convert said imino halide compound to the hydrohalide salt of the corresponding imino ether compound; d. converting said imino ether compound by hydrolysis at a temperature below about -20°C to the hydrohalide salt of a compound of the formula: ##SPC24## e. reacting a compound formed in step (d) with an acylating agent selected from an organic carboxylic acid and a functional reactive derivative of such acid in the presence of an acid binding agent to obtain acylation of the amino group; and (f) treating said acylated compound with sufficient water to obtain a compound of the following formula: ##SPC25##and the corresponding acid addition salt where a free amino group is present on the acyl radical, wherein: M is selected from the class consisting of hydrogen, an alkali metal and a tertiary amine; R¹⁵ is a substituent selected from those contained in a natural and semi-synthetic penicillin; R¹ and R² are selected from the class consisting of (lower)alkoxy, (lower)alkylthio, phenyl, naphthyl, phenoxy, naphthoxy, phenylthio, phenyl(lower)alkyl, phenyl(lower)alkylthio, halogen, lower alkyl, halo(lower)alkyl, halo(lower)alkyloxy, a radical of the formula ##SPC26##R¹ and R² when joined together form with the phosphorus atom the ring ##SPC27## R³ is the oxygen atom which when present is linked by a double bond to the phosphorus atom; X is selected from the class consisting of oxygen, sulfur and methylene; R⁴ is selected from the class consisting of hydrogen and (lower)alkyl; n is an integer from 0 to 1; m is an integer from 1 to
 6. 5. A process according to claim 4 wherein R¹⁵ is a substituent contained in a natural penicillin.
 6. A process according to claim 5 wherein R¹⁵ is selected from the class consisting of benzyl and phenoxymethyl.
 7. A process according to claim 6 wherein said phosphorylating agent is a compound selected from those of the formula: ##SPC28##
 8. A process according to claim 7 wherein said acyl radical is selected from the base consisting of those having the following formulae: ##SPC29##wherein: R⁶ and R⁷ are selected from the group consisting of hydrogen and (lower)alkoxy; R⁸ is selected from the group consisting of hydrogen, (lower)alkyl and phenyl; R⁹ and R¹⁰ are selected from the group consisting of hydrogen and halogen; R¹¹ and R¹² are selected from the group consisting of hydrogen, halogen, (lower)alkyl, (lower)alkoxy, phenyl and phenoxy; R¹³ is selected from the class consisting of hydrogen, (lower)alkyl and aryl; a is an interger from 0 to 1; b is an interger from 0 through 5; c is an interger from 0 to 2; d is an interger from 1 through 3, with the proviso that when a is 0, d is greater than 1, and when a is 1, d is less than
 3. 9. A process according to claim 5 wherein said phosphorylating agent is phosphorus trichloride.
 10. A hydrohalide salt of the formula: ##SPC30##wherein: R¹ and R² are selected from the class consisting of (lower)alkoxy, (lower)alkylthio, phenyl, naphthyl, phenoxy, naphthoxy, halo, phenylthio, phenyl(lower)alkyl, phenyl(lower)alkylthio, phenyl(lower)alkyloxy, (lower)alkyl, halo(lower)alkoxy, a radical of the formula ##SPC31##R¹ and R² when joined together form with the phosphorus atom the ring ##SPC32##
 11. The hydrohalide salt of the compound of the formula: ##SPC33##wherein R⁴ is selected from the class consisting of hydrogen and lower alkyl; and m is an integer from 1 to
 6. 12. A compound according to claim 11 which is the hydrochloride salt of 6-aminopenicillanic acid, 1,3,2-dioxaphospholan-2-yl ester.
 13. A compound according to claim 11 which is the hydrochloride salt of 6-aminopenicillanic acid, 1,3,2-dioxaphosphorinan-2-yl-ester.
 14. A compound according to claim 10 which is the hydrochloride salt of 6-aminopenicillanic acid anhydride with phosphorodichloridus acid. 